DETEKSI BAKTERI ENTEROPATOGENIK PADA SUMBER AIR DAN AIR MINUM DI YOGYAKARTA

Sarlen Sihombing; Tri Yahya Budiarso

Sarlen Sihombing
Tri Yahya Budiarso

Keywords: Enteropatogenik, air minum, Proteus mirabillis, Klebsiella pneumonia

Abstract

Water is important for humans life especially for drinking, cooking, and washing. The water
should be free from contaminations of enteropathogenic bacteria so it does not cause
diseases.The purpose of this study was done to isolate and identify contamination of
enteropathogenic bacteria in water from the well, local drinking water companies (PDAM),
and bottled drinking water (AMDK). Isolation was done by using Chromucult Coliform Agar
(CCA) and Salmonella Shigella Agar (SSA) as a selective and differential media. Predictable
colonies of enteropathogenic were selected with biochemical test and confirmed by API 20 E.
The result showed that ten percent from total sample tested from well water and bottle water
contaminated by enteropathogenic bacteria. The suspect’s enteropathogenic colonies were
identified as Proteus mirabillis with a percentage %ID values of 99.9% and 99.2%. The result
also proved that ten percent samples from the bottled drinking water contamined with
bacteria. Based on API 20E test, the suspect’s colonies were identified as Klebsiella
pneumonia (ID value : 98.5% and 98.0%).
Keywords : Enteropathogenic, Drinking Water, Proteus mirabills, Klebsiella pneumonia

References

Brooks GF. Janet SB., Stepens, AM. 2007. Mikrobiologi Kedokteran. Edisi 23. Alih bahasa Hartanto et al. Jakarta : EGC.
Budiarso, T.Y and Belo, MJX. 2009 Deteksi Cemaran Salmonella sp pada Daging Ayam yang dijual di Pasar Tradisional di Wilayah Kota Yogyakarta, 245–251; prosiding seminar nasional penelitian, pendidikan dan penerapan MIPA, Fakultas MIPA, Universitas Negeri Yogyakarta, 16 Mei 2009. h.B-246
Cabral JPS. 2010 Water microbiology, bacterial pathogens and water, International Journal of Environmental Research and Public Health, 7(10) : 3657–3703. doi: 10.3390/ijerph7103657.
Centers For Disease Control and Prevention/ 20132013 Emergency Management Program Activities — Worldwide. 62(35). 2011–2012.
Jawetz, Melnick, and Adelberg’s. 2001 Medical Microbiologi, ed 20. Jakarta: Penerbit Salemba Medika;. h. 224-227
Manos J and Belas R. 2006. The Genera Proteus, Providencia, and Morganella. Chapter 3.3.12, 10.1007/0-387-30746-x_12
O’Hara CMO., Brenner FW., and Miller JM. 2000 Classification , Identification , and Clinical Significance of Proteus , Providencia , and Morganella. Journal Clinical Microbiology Reviews, 13(4) 534–546.
Swihartt KG., and Welch RA. 1990 The HpmA Hemolysin Is More Common than HlyA, Infection and Microbiology, among Proteus Isolates, 58(6):1853–1860.
Talaro KP, Talaro A 2000. Drugs, microbes, host-The elements of chemotherapy. In: Foundations in Microbiology. 4th ed. McGraw-Hill, New York, 348-379.
Turner KM., Restaino L. and Frampton EW. 2000. Efficacy of chromocult coliform agar for coliform and Escherichia coli detection in foods. Journal of Food Protection, 63(4) : 539–541.